Oestradiol-17 beta influence on testosterone production of luteal cells from early pregnant, oestradiol benzoate-treated and human chorionic gonadotropin-treated sows in vitro
Grazul, A.; Przała, J.; Wiesak, T.; Muszyńska, A.
Acta Veterinaria Hungarica 34(3-4): 263-269
1986
ISSN/ISBN: 0236-6290 PMID: 2953212 Document Number: 279472
Luteal cells separated from early pregnant, oestradiol benzoate- (EB) treated and HCG treated sows were incubated with or without oestradiol-17 beta (E2) to estimate the influence of E2 on testosterone (T) production in vitro. The concentrations of E2 were 1, 10, 100, 1000 and 5000 ng/ml medium. Trypsin-dispersed luteal cells, 5 x 104 cells/ml, were suspended in Eagle's medium enriched with 2% human serum albumin. The T amounts in the medium samples were estimated by radioimmunoassay after an incubation of 1, 3, and 6 h. The luteal cells from the three groups of sows released similar amounts of T during incubation: 82.5 +or- 10.9, 61.4 +or- 7.1 and 85.2 +or- 13.0 pg/ml, respectively. The cells from pregnant pigs reduced T secretion in the presence of 10 ng E2 per ml medium in the first hour of incubation and increased it in the presence of the highest E2 concentration in the third hour of the experiment. E2 did not change the T production of luteal cells obtained from the remaining two groups (EB- and HCG-treated sows, respectively).