On the relative biological and immunoreactive potencies of luteinizing hormone in the oestradiol benzoate-treated male rat
Grotjan, H.E.; Steinberger, E.
Acta Endocrinologica 89(3): 538-550
1978
ISSN/ISBN: 0001-5598 PMID: 707009 Document Number: 124417
Mature male Sprague-Dawey rats were treated with 50 .mu.g estradiol benzoate (EB) per day for 7 days and killed 24 h after the last injection. Serum and pituitary luteinizing hormone (LH) levels were measured by RIA rLH-I4. Chronic EB-treatment decreases pituitary LH and suggests a decrease in serum LH as well. Comparison of the present with previous results revealed particular combinations of RIA reagents yield divergent estimates for serum LH in EB-treated male rats. This situation emphasizes the need to carefully specify all RIA reagents used in any study since the results may vary with different lots of RIA reagents.