Simultaneous determinations of oestradiol-17 beta, progesterone and luteinizing hormone in the plasma during the ovulatory cycle of the hen
Shodono, M.; Nakamura, T.; Tanabe, Y.; Wakabayashi, K.
Acta Endocrinologica 78(3): 565-573
1975
ISSN/ISBN: 0001-5598 PMID: 1173019 Document Number: 90794
Blood samples were collected from the wing vein of 7 hens with regular ovulatory cycles at intervals of 1, 2 or 4 h throughout a 36-h period starting 24-25 h before and ending 11-12 h after ovulation of the 2nd egg of the clutch. The oestradiol-17 beta level of the plasma during the ovulatory cycle varied from 50 to 250 pg/ml. The value was high 24 h before ovulation and decreased until 10 h before ovulation, then increased and reached a peak 4-5 h before ovulation, when it started to decrease again until 12 h after ovulation. The plasma levels of progesterone varied from 1 to 6 ng/ml, and reached a peak 4-6 h before ovulation. The plasma LH value varied from 1 to 4 ng/ml, and the changes in the level corresponded well with that of progesterone, peak values being coincident.