Acquisition and aggregation of canine blood platelets: basic mechanisms of function and differences because of breed origin
Clemmons, R.M.; Meyers, K.M.
American Journal of Veterinary Research 45(1): 137-144
1984
ISSN/ISBN: 0002-9645 PMID: 6422804 Document Number: 239562
Platelets were separated from plasma by adding sodium citrate. Maintaining a quiet environment or anaesthetizing the animals with thiamylal sodium aids collection of platelets. Aggregation of platelets from 60 dogs of various breeds in response to arachidonic acid, collagen, adenosine diphosphate, epinephrine, and serotonin was monitored. Canine platelets reversibly or irreversibly aggregated to arachidonic acid. The percentage of arachidonate-irreversible platelets varied from 0 to 100, depending on breed. Arachidonate-irreversible platelets also aggregated at low concentrations of collagen and exhibited biphasic irreversible aggregation to adenosine diphosphate and serotonin. Serotonin-induced irreversible aggregation was dependent upon receptor activation and upon arachidonic acid metabolism. Irreversible aggregation to serotonin was associated with release of superscript 3H-serotonin and thromboxane B2 formation, indicating a link between the serotonergic receptor and arachidonic acid metabolism.