Top Abstract Purchase Related
Home
>
Node
>
Subnode

Trypanosoma cruzi: description of a highly purified surface antigen defined by human antibodies

Scharfstein, J.; Rodrigues, M.M.; Alves, C.A.; de Souza, W.; Previato, J.O.; Mendonça-Previato, L.

Journal of Immunology 131(2): 972-976

1983

ISSN/ISBN: 0022-1767
PMID: 6345675
Document Number: 219301
A glycoprotein of molecular weight 25 000 (G25) purified from T. cruzi extracts was recognized by serum antibodies of Chagas' disease patients. These human antibodies were isolated by affinity chromatography and were used to demonstrate that G25 antigenic determinants are represented at the parasite surface, and are expressed in all developmental stages of the parasite's life-cycle, as well as in several T. cruzi strains. Antibodies to radiolabelled G25 were found in the serum of 96.5% of 173 Chagasic patients from different endemic areas, but were not found in the serum from other individuals. Taken collectively, the data suggest that antibodies to G25 define highly conserved determinants of T. cruzi.

Document emailed within 1 workday
Secure & encrypted payments

Ortaldo, J.R.; Sharrow, S.O.; Timonen, T.; Herberman, R.B. 1981: Determination of surface antigens on highly purified human NK cells by flow cytometry with monoclonal antibodies Journal of Immunology 127(6): 2401-2409
Ouaissi, M.A.; Taibi, A.; Loyens, M.; Martin, U.; Afchain, D.; Maidana, C.; Caudioti, C.; Cornette, J.; Martelleur, A.; Velge, P. 1991: Trypanosoma cruzi: a carbohydrate epitope defined by a monoclonal antibody as a possible marker of the acute phase of human Chagas' disease American Journal of Tropical Medicine and Hygiene 45(2): 214-225
Katzin, A.M.; Del Pino, E.J.; Cunio, R.M.; Raisman, J.S.; Olmos, J.; Lajmanovich, S.; González Cappa, S.M. 1979: Lectin receptors on the surface of Trypanosoma cruzi epimastigotes Medicina 39(1): 76-84
González, M.J.; López-Solís, R.; Coloma, L.; Toro, G.C.; Galanti, N. 1988: Exposure of sialic acids at the surface in Trypanosoma cruzi epimastigotes Archivos de Biologia Y Medicina Experimentales 21(3-4): 461-465
Sánchez, D.O.; González Cappa, S.M. 1983: Neutralizing antibodies in Trypanosoma cruzi infection Medicina 43(1): 41-46
Meirelles, M.N.; Souto-Padrón, T.; De Souza, W. 1984: Participation of cell surface anionic sites in the interaction between Trypanosoma cruzi and macrophages Journal of Submicroscopic Cytology 16(3): 533-545
Celis, E.; Ou, D.; Otvos, L. 1988: Recognition of hepatitis B surface antigen by human T lymphocytes. Proliferative and cytotoxic responses to a major antigenic determinant defined by synthetic peptides Journal of Immunology 140(6): 1808-1815
Juri, M.A.; Ferreira, A.; Ramos, A.; Hoecker, G. 1990: Non-lytic antibodies in H-2-controlled resistance to acute infection with Trypanosoma cruzi Brazilian Journal of Medical and Biological Research 23(8): 685-695
Aguilar-Torres, F.G.; Rytel, M.W.; Kagan, I.G. 1976: Comparison of counterimmunoelectrophoresis (CIE) with other serologic tests in the detection of antibodies to Trypanosoma cruzi American Journal of Tropical Medicine and Hygiene 25(5): 667-670
Lissaldo, A.M.; Hoshino-Shimizu, S.; Umezawa, E.S.; Stolf, A.M. 1994: Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to Dot-ELISA Revista do Instituto de Medicina Tropical de Sao Paulo 36(2): 163-166
Pinto, C.; Miguel; Grijalva, M.J.; Costales, J.A. 2003: Prevalence of Trypanosoma cruzi in rodents and marsupials of two localities of Manabi, Ecuador Prevalencia de Trypanosoma cruzi en roedores y marsupiales en dos localidades de Manabi, Ecuador Revista de la Pontificia Universidad Catolica del Ecuador. Septiembre; 71: 225-233
Altcheh, J.; Corral, R.; Biancardi, M.A.; Freilij, H.éc. 2003: Anti-F2/3 antibodies as cure marker in children with congenital Trypanosoma cruzi infection Medicina 63(1): 37-40
Pereira, C.A.; Longo, I.M.; Ricci, O.; de Paiva e Silva, A. 1980: Automated complement fixation test for the detection of anti-trypanosoma cruzi antibodies Revista do Instituto de Medicina Tropical de Sao Paulo 22(4): 180-183
Gil, J.é; Cimino, R.én.; López Quiroga, I.és.; Cajal, S.; Acosta, N.; Juarez, M.; Zacca, R.; Orellana, V.; Krolewiecki, A.; Diosque, P.; Nasser, J. 2011: Reactivity of GST-SAPA antigen of Trypanosoma cruzi against sera from patients with Chagas disease and leishmaniasis Medicina 71(2): 113-119
Tibbetts, R.S.; McCormick, T.S.; Rowland, E.C.; Miller, S.D.; Engman, D.M. 1994: Cardiac antigen-specific autoantibody production is associated with cardiomyopathy in Trypanosoma cruzi-infected mice Journal of Immunology 152(3): 1493-1499
Galavíz-Silva, L.; Molina-Garza, D.P.; González-Santos, M.A.; Mercado-Hernández, R.; González-Galavíz, Jé.Reyes.; Rosales-Encina, Jé.Luis.; Molina-Garza, Z.J. 2009: Update on seroprevalence of anti-Trypanosoma cruzi antibodies among blood donors in northeast Mexico American Journal of Tropical Medicine and Hygiene 81(3): 404-406
Nikolaev, I.S.; Gil'chuk, P.V.; Gorbatiuk, O.B.; Fliak, A.I.; Labyntsev, A.I.; Irodov, D.M.; Kolibo, D.V.; Kordium, V.A. 2011: Polyclonal antibodies against human cell-surface antigen CD34 Tsitologiia i Genetika 45(3): 3-14
Pereira, C.A.; Mimica, I.M.; Takeda, A.K. 1980: Automated agglutination reactions for the detection of Australia antigen, antipolysaccharide antibodies of T. cruzi and anti-Salmonella flagellar antibodies Boletin de la Oficina Sanitaria Panamericana. Pan American Sanitary Bureau 88(2): 119-126
Reis, D.D.; Gazzinelli, R.T.; Gazzinelli, G.; Colley, D.G. 1993: Antibodies to Trypanosoma cruzi express idiotypic patterns that can differentiate between patients with asymptomatic or severe Chagas' disease Journal of Immunology 150(4): 1611-1618
Baschenko, I.A.; Khavkin, I.A. 1986: The use of highly purified staphylococcal alpha-toxin in the immunochemical purification of antibodies Voprosy Meditsinskoi Khimii 32(4): 27-32