The use of highly purified staphylococcal alpha-toxin in the immunochemical purification of antibodies
Baschenko, I.A.; Khavkin, I.A.
Voprosy Meditsinskoi Khimii 32(4): 27-32
1986
ISSN/ISBN: 0042-8809 PMID: 3532530 Document Number: 273109
A simple procedure is described for isolation of highly purified staphylococcal alpha-toxin (SAT) by means of sorption on macroporous glass beads and gel filtration. Various conditions were tested of affinity immunosorption of antibodies and SAT. Highly-porous polyacrylamide gel activated by glutaraldehyde was found as a readily available and selective immunosorbent. The immobilized antigen was stabilized using reduction of azomethyne bonds. Specific activity of antibodies was elevated as the SAT purification degree increased. Correlation between antihemolytic and antilethal activities was unaltered during the immunochemical purification of antibodies.