Preparation of highly purified cephamycin C
Kunishima, M.; Yabuuchi, M.; Masuda, M.; Imazumi, K.; Hamano, H.
Japanese Journal of Antibiotics 35(7): 1699-1700
1982
ISSN/ISBN: 0368-2781 PMID: 7176069 Document Number: 193224
Preparation of highly purified cephamycin C (CM-C) is described in this report. The purification of CM-C was carried out by Sephadex LH-20 chromatography of the N-BOC-CM-C developed with MeOH followed by removal of the protecting group. Partially purified CM-C was further refined by Sephadex LH-20 chromatography developed with MeOH and Diaion HP-20 SS chromatography developed with H2O. CM-C thus obtained showed the UV absorption at 265 nm [E1% 1cm 184.4 (H2O)] which is the strongest absorption ever appeared in the literatures.