Automated agglutination reactions for the detection of Australia antigen, antipolysaccharide antibodies of T. cruzi and anti-Salmonella flagellar antibodies
Pereira, C.A.; Mimica, I.M.; Takeda, A.K.
Boletin de la Oficina Sanitaria Panamericana. Pan American Sanitary Bureau 88(2): 119-126
1980
ISSN/ISBN: 0030-0632 PMID: 6445198 Document Number: 155388
This article describes the development and testing of automated systems for detecting Australia antigen, antibodies against T. cruzi and anti- Salmonella flagellar antibodies. The basic techniques used were passive hemagglutination in the first 2 instances and bacterial agglutination in the third. All 3 procedures employed a modified version of automated equipment currently used to detect syphilis. The new automated methods provided specific, easily readable and rapid results. These trials were conducted with sera from 50 blood donors for detection of Australia antigen, sera from 60 Chagas' disease patients and 14 control subjects for detection of antibody against T. cruzi, and sera from 197 blood donors for detection of anti-Salmonella flagellar antibodies. The new techniques offer definite advantages if large numbers of samples are processed and they seem well-suited to use in blood bank screening activities and epidemiologic investigations.