Monoclonal antibodies to Rho (D) -antigen of the Rhesus system. Obtaining the EBV-transformed B-lymphoblastoid human cell lines secreting IgG-anti-D antibodies
Deriugina, E.I.; Olovnikova, N.I.; Lemeneva, L.N.; Belkina, E.V.; Udalov, G.A.; Chertkov, I.L.
Gematologiia i Transfuziologiia 36(8): 11-16
1991
ISSN/ISBN: 0234-5730 PMID: 1661249 Document Number: 375555
Human B-lymphoblastoid cell lines producing specific anti-D antibodies against Rh0(D) antigen have been established by EBV-transformation. The strong influence of the donor's immune status (serum titer of anti-D antibodies, intravenous booster injection prior to bleeding) is shown. The cell lines cloned by limiting dilution continuously produce anti-D antibodies in culture for 7 months or longer which is sufficient to support large-scale production of anti-D antibodies based on the cultivation of cryopreserved cells. Anti-D IgGl monoclonal antibodies obtained react with Rh-positive and Du cells but not with Rh-negative cells in serological tests. These human monoclonal antibodies could be useful in the prevention of Rh hemolytic disease of newborns.