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Enhancement of reactivity of HLA-Cw antisera by extending the incubation time

Wong, D.M.; Kumar, R.; Mittal, K.K.

Acta Anthropogenetica 7(3): 197-203

1983

ISSN/ISBN: 0258-0357
PMID: 6335043
Document Number: 210720
HLA-Cw antisera showing weak cytotoxic activity when tested at 1 1/2 hours in the conventional HLA-A,B, Cw testing procedure, were found to react better in the test using an extended incubation period of 3 hours. Of the 71 sera which were tested, 15 (21%) sera demonstrated an increase in anti-HLA-Cw reactivity against cells from donors possessing the corresponding Cw antigen. In addition, the use of B cells in the testing procedure appeared to be more favorable than T cells in the enhancement of reactivity of weak HLA-Cw antisera.

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