Determination of the MSF (macrophage slowing factor) release by phytohemagglutinin using the macrophage electrophoretic mobility test (MEM test) . I. Dependency of release on incubation time, phytohemagglutinin and cell concentration
Metzner, G.; Jäger, L.
Acta Biologica et Medica Germanica 39(2-3): 223-227
1980
ISSN/ISBN: 0001-5318 PMID: 6999821 Document Number: 163273
Under the influence of phytohaemagglutinin (PHA) lymphocytes of the peripheral blood from healthy persons and patients without immunodeficiencies produce a lymphokine called macrophage slowing factor (MSF). Kinetic studies of MSF production according to time-dependence, dose-response-effect and lymphocyte concentration revealed the optimal test conditions: 90 min resp. 4 h incubation time, 1 microliter and 5 microliter PHA per test and 10(6) lymphocytes per test. These conditions provided a decrease of 11.0 +/- 4.1% of macrophage mobility in the electric field. The PHA-model can be used as a positive control for the MEM-test. Moreover the PHA-model serves as a test for immunological reactivity.