Comparative study of the proliferative activity of native and cryopreserved colony-forming units in humans and mice
Tsutsaeva, A.A.; Drozdova, O.A.; Ostankova, L.V.; Mikulinskiĭ, I.E.; Gol'tsev, A.N.
Tsitologiia i Genetika 16(1): 14-18
1982
ISSN/ISBN: 0564-3783 PMID: 7064219 Document Number: 195814
The agar culture method was used to state that colony-forming units responsible for cell structures of a hemopoietic series remained undamaged after cryopreservation (-196 degrees) in a suspension of mouse and human donor bone marrow. Cultivation of cryopreserved bone marrow revealed the inhibition of proliferative activity of cells growing in agar as compared with native ones. The inhibition of DNA synthesis was the basis of this process.