Stability of the biocatalysts of L-aspartic acid synthesis based on immobilized Escherichia coli cells
Zueva, N.N.; Iakovleva, V.I.; Avsiuk, I.V.; Arens, A.K.; Fechina, V.A.
Prikladnaia Biokhimiia i Mikrobiologiia 18(5): 681-687
1982
ISSN/ISBN: 0555-1099 PMID: 6755439 Document Number: 191803
Experiments were carried out to investigate the process of a continuous enzymatic synthesis of L-aspartic acid from ammonium fumarate in uniform filling flow reactors. Escherichia coli (Soviet strain 85) cells immobilized in polyacrylamide gel granules reinforced by a solid carrier were used as biocatalysts. The conditions, under which a high aspartase activity of the biocatalyst and a stable hydrodynamic performance of the reactor were maintained, were determined. The main kinetic characteristics of a continuous performance of the reactor for 150 days were obtained.