Maintenance stability of the pBR322 and pBR327 vector plasmids in Escherichia coli cells during multiple passages
Potapova, I.A.; Repin, V.E.; Shchelkunov, S.N.
Genetika 21(2): 344-346
1985
ISSN/ISBN: 0016-6758 PMID: 3886482 Document Number: 266010
Stability of pBR322 and pBR327 plasmids was studied. Plasmid-containing Escherichia coli strains were grown in liquid growth medium without selection pressure. Plasmid pBR327 was shown to be more stable in E. coli CSH54 cells than pBR322. Essential heterogenity of individual plasmid-containing clones was recognized by the maintenance stability of plasmid DNA. The indicated clones with high stability failed to be cured from pBR327 plasmid by means of acridine orange. High stability of plasmid maintenance and the failure to cure cells containing this plasmid are suggested to correlate with and to be essentially determined by the cell functions.