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Synthesis of oligoribonucleotides with the use of RNA polymerases of E. coli and immobilized synthetic DNA-templates

Denisova, L.I.; Zagrebel'nyy, S.N.; Pustoshilova, N.M.; Ven'iaminova, A.G.; Gorn, V.V.

Bioorganicheskaia Khimiia 15(4): 562-565

1989

ISSN/ISBN: 0132-3423
PMID: 2665754
Document Number: 337980
A new approach to synthesis of oligoribonucleotides is suggested, based on transcription by E. coli RNA polymerase of synthetic immobilized DNA-templates with AUG as primer. The approach has been experimentally verified by synthesis of two oligonucleotides, viz., a RNA fragment of the fr phage (16 nucleotides long) and a RNA fragment of the tickborne encephalitis virus (18 nucleotides long). Fraction of the synthesized RNA fragments in the whole nucleotide material is about 20%. The templates can be used repeatedly. Sequences of the oligoribonucleotides were confirmed. Advantages of this approach and its usefulness for SP6 DNA-dependent RNA polymerase are discussed.

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