New methodology for the synthesis of 2' (3') -O-aminoacyl oligoribonucleotides related to the 3'-terminus of aa-tRNA
Hagen, M.D.; Scalfi-Happ, C.; Happ, E.; Chládek, S.
Nucleic Acids Symposium Series 18: 285-288
1987
ISSN/ISBN: 0261-3166 PMID: 3697143 Document Number: 299663
A new synthetic approach to the title compounds is reported. It is based on the phosphotriester methodology and uses a unique combination of protecting groups (Fmoc and Ph for the aglycons; DMT, CPTr and Mthp for the carbohydrate hydroxy functions; 2-CIPh for the phosphodiester bonds; BPOC for amino acid). This enables selective deprotection of the blocked oligonucleotide intermediates in two steps (oximate and acid treatments) to yield the 2'(3')-O-aminoacyl oligoribonucleotides suitable for biochemical investigations.