Comparison of proliferation and cytopathogenicity of swine vesicular virus and coxsackievirus B5
Lai, S.S.; Breese, S.S.; Moore, D.M.; Gillespie, J.H.
Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi 14(3): 167-172
1981
ISSN/ISBN: 0253-2662 PMID: 6269809 Document Number: 171895
The sequential appearance of both swine vesicular disease (SVD) virus and coxsackievirus B5 antigens in a pig kidney cell line was studied by immunofluorescence and electron microscopy. The replication cycle of each virus was approximately 3-4 hours. Viral antigens were demonstrable in the cytoplasm 2 hours after inoculation. A compact mass of fluorescence was seen when cells showed cytopathic effect at 5.5 hours. After 3 hours, a few viral particles, observed by electron microscopy, were in the cytoplasm. Morphological changes in the cells occurred at the same time. Cytoplasmic crystalline arrays of virus were first detected at 7 hours. The findings support the view that SVD and B5 viruses are closely related.