Controlled cell cultivation. IV. the cultivation of isolated neurons of the mollusc, Limnaea stagnalis, in a flow-diffusion chamber
Arkhipov, V.V.; Lezhnev, E.I.; Veprintsev, B.N.
Tsitologiia 22(5): 606-610
1980
ISSN/ISBN: 0041-3771 PMID: 7434471 Document Number: 156686
A possibility of the long-term cultivation of isolated neurons of adult molluscs Limnaea stagnalis in a flow-diffusion chamber has been shown. The construction of the chamber is described by the authors elsewhere; the diagram of the pefusion apparatus has been presented. In the process of cultivation, the nerve cells undergo morphological differentiation to form neuron nets on the glass that involve both individual neurons and their aggregates of 2 to 5 cells. By days 16-18 of cultivation, most of the neurons join together to form several large aggregates of 10-40 cells, connected to each other by thick nerve fibre bundles. A sharp decrease of survival time and the complete inhibition of morphological differentiation of neurons was observed when the diffusional exchange between the extracelluar space and the flowing nutrient medium was replaced in the chamber by the medium perfusion through the extracellular (=pericellular) space. The cause of this phenomenon is discussed.