Cultivation of murine B-cell hybridomas in the spleen
Volgareva, G.M.; Kil'diushevskaia, T.V.; Del'vig, A.A.; Kozhevnikova, E.V.; Sviridov, V.V.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 1: 94-98
1987
ISSN/ISBN: 0372-9311 PMID: 3551420 Document Number: 302747
The tumorigenic capacity of mouse B-cell hybridomas in both cloned and primary cultures was studied. The cells were selected for inoculation from 24-well plates and introduced into the spleen of syngeneic mice. The cells took in 50% of the animals. The cells of hybridoma tumors induced as the result of intrasplenic inoculation, when subcultured in the second passage following the standard scheme, i.e. inoculated intraperitoneally in a dose of 1 X 10(7) cells into mice previously treated with vaseline oil or pristane, produced tumors in 100% of the animals.