Studies on the cultivation of isolated murine renal glomeruli (author's transl
Mahnke, P.F.; Schulze, S.
Zentralblatt für Allgemeine Pathologie U. Pathologische Anatomie 121(4-5): 456-464
1977
ISSN/ISBN: 0044-4030 PMID: 919829 Document Number: 118450
The growth rate of isolated murine renal glomeruli was studied in vitro. Separation by screening was followed by cultivation in Carrel flacks using Eagle's medium and the number of utilizable tests was 104. It was possible to cultivate glomeruli for up to 27 days and to observe cell growth. There were epithelial capsule cells, endothelial capillary cells and fibroblasts. The existence of mesangial cells was not convincingly demonstrated. The problems associated with the cultivation of isolated renal glomeruli were discussed. Possible uses of this particular model were discussed.