Cultivation of Mycobacterium tuberculosis in cell culture
Alekseev, A.I.; Durymanov, A.G.; Rassadkin, I.N.; Repin, V.E.; Shestopalov, A.M.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 4: 63-65
2004
ISSN/ISBN: 0372-9311 PMID: 15481925 Document Number: 575640
The method of the cultivation of M .tuberculosis in cell subcultures was tested. Five consecutive passages of 2 M. tuberculosis strains were carried out with similar inoculation and cultivation conditions. Mycobacterial cells preserved their morphological characteristics in the course of all passages. The method of M. tuberculosis cultivation in subcultured cells made it possible to study of the physiology of M. tuberculosis under conditions, most approximated to the natural tuberculosis infection in humans.