Dynamics of the amino acid composition of the medium during cultivation of isolated organs by the directed perfusion method
Barashkov, V.A.; Gitel'zon, I.I.; Nefedov, V.P.; Trubachev, I.N.
Biulleten' Eksperimental'noi Biologii i Meditsiny 80(7): 36-38
1975
ISSN/ISBN: 0365-9615 PMID: 1227646 Document Number: 85536
The dynamics of the amino acid composition of the medium under conditions of adequate perfusion of the isolated organs of a dog (sternum, kidney and liver) was studied. It was found that after a 6-hour perfusion of the complex of organs the amount in the perfusion medium of such amino acids as histidine, lysine, alanine, considerably increased, whereas the amount of arginine, serine, aspartic acid, threonine with glutamine, isoleucine, proline, leucine and valine decreased as compared with their initial concentration. The dynamics of the amino acid medium composition during a 4-hour perfusion was studied in experiments with the isolated sternum. The concentration of alanine, lysine and histidine increased in the medium. At the same time there was seen a decrease in the concentration of serine, aspartic acid, isoleucine, tyrosine and phenyl-alanine.