Determination of the amino acid composition of cartilage and creatine phosphokinase isoenzymes with the use of phenylthiocarbamyl amino acid derivatives
Romantsev, F.E.; Prozorovskiĭ, V.N.; Kozlov, E.A.; Karpov, D.A.; Trebukhina, E.L.
Voprosy Meditsinskoi Khimii 35(3): 71-75
1989
ISSN/ISBN: 0042-8809 PMID: 2773389 Document Number: 340659
A method for estimation of amino acid composition and collagen-specific amino acids was developed. The assay was based on the precolumn reaction of amino acids with phenylisothiocyanate. The phenylthiocarbamyl derivatives were analyzed by means of reverse-phase chromatography on octadecyl sorbents, which involved gradient elution with ethanol and sodium or ammonium acetate. The method was used for effective separation of collagen-specific and main amino acids in cartilage hydrolysate from healthy persons and of patients with funnel chest and Ehlers-Danlos syndrome and also for determination of amino acid composition in creatine phosphokinase B- and M-subunits.