Chemokinetic response of activated macrophages to soluble products of neoplastic cells
Snodgrass, M.J.; Harris, T.M.; Kaplan, A.M.
Cancer Research 38(9): 2925-2929
1978
ISSN/ISBN: 0008-5472 PMID: 679201 Document Number: 129408
The rate of lateral movement of normal (unelicited) and pyran-activated murine (C57BL/6) peritoneal macrophages was used as a parameter of the macrophage response to exposure to the soluble by-products of neoplastic cells. Macrophages suspended in nutrient medium (5 .times. 105 cells/ml) were injected into Sykes-Moore chambers, and their movement was recorded by time lapse cinematography for 37 h. After 14 h of filming, the nutrient medium was removed and replaced with fresh nutrient medium or cell-free conditioned media from syngeneic Lewis lung carcinoma of 2182 fibrosarcoma cells or syngeneic mouse embryo fibroblasts. Activated macrophages in nutrient medium moved about 2.0 times faster than did normal macrophages. When the medium was replaced with cell-free medium from the nonneoplastic mouse embryo fibroblasts, the rate of movement did not change. Cell-free conditioned media from the neoplastic cell lines caused a 2- to 3-fold increase in the rate of lateral movemet of pyran-activated macrophages. The conditioned media did not induce any rate change when added to normal macrophages. Thus, activated macrophages responded cytotropically to soluble byproducts of the neoplastic cells tested.