Augmentation of cytotoxicity of lymphokine activated killer cells on ovarian tumor cells by various biological response modifiers
Nio, Y.; Zighelboim, J.; Berek, J.S.; Bonavida, B.
Anticancer Research 10(2a): 441-446
1990
ISSN/ISBN: 0250-7005 PMID: 2346317 Document Number: 353766
The effect of combining lymphokine activated killer (LAK) cells with either recombinant interleukin - 1 (rIL-2), recombinant interferon - alpha (rIFN-.alpha.), recombinant tumor necrosis factor (rTNF) or streptococcal preparation OK-432 were assessed, using Raji, 2 kinds of cultured ovarian lines (PA-1 and SKOV - 3), and 7 fresh ovarian tumors lines. LAK cells were generated by culturing peripheral blood lymphocytes (PBL) with rIL-2 for 3 -5 days. The simultaneous combination of LAK cells with rIFN.alpha. or OK-432 augmented the cytotoxicity of LAK cells. The susceptibility of tumor cells to LAK cells also increased after the pretreatment of tumor cells to LAK cells also increased after the pretreatment of tumor cells with OK-432. These results suggest that the simultaneous injection of LAK cells with rIFN-.alpha. or OK-432 and the intralesional injection of OK-432 prior to the adoptive transfer of LAK cells may be a beneficial combination treatment for LAK treatment.