Evaluation of the effectiveness of receptor destroying enzyme preparations
Styk, B.; Russ, G.; Varecková, E.
Acta Virologica 21(6): 474-484
1977
ISSN/ISBN: 0001-723X PMID: 23666 Document Number: 118809
A procedure has been developed for testing receptor destroying enzyme (RDE) preparations used to remove nonspecific inhibitors before carrying out haemagglutination inhibition tests with influenza virus. Four criteria should be taken into account: (1) titre of RDE, usually used to indicate the activity of the preparation; (2) neuraminidase activity of the preparation, determined biochemically, which partially corresponds to the RDE titre; (3) direct demonstration of the complete removal of nonspecific inhibitors; and (4) determination that the RDE preparation does not affect specific antibody.