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Isolation and properties of preparations the branching enzyme of muscles, amylose isomerase

Shvedova, T.A.; Petrova, A.N.

Prikladnaia Biokhimiia i Mikrobiologiia 14(5): 683-689

1978

ISSN/ISBN: 0555-1099
PMID: 30978
Document Number: 125936
The paper describes a modified method of isolating the branching enzyme of amylose isomerase from muscles and a study of the enzyme activity at different stages of purification. By enzyme fractionation on biogel R-150 and Sepharose 6B the fractions containing different RNA amounts have been isolated. The activity of fractions has been shown to depend on their content of RNA. The paper presents a procedure used to isolate a highly purified fraction of amylose isomerase and its properties (pH and temperature optima, enzyme optimal concentration and Michaelis constant).

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