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Isolation and properties of the fibrinolytic enzyme from the Actinomyces thermovulgaris cultural broth

Egorov, N.S.; Kochetov, G.A.; Khaĭdarova, N.V.

Mikrobiologiia 45: 455-459

1976

ISSN/ISBN: 0026-3656
PMID: 12451
Document Number: 105858
A fibrinolytic enzyme was isolated from the cultural broth of Actinomyces thermovulgaris T-54 by precipitation with ammonium sulphate to 0.8 saturation and chromatography on GE-cellulose and CM-cellulose. The enzyme is homogeneous as was confirmed by disk electrophoresis in polyacrylamide gel. Some properties of the enzyme were studied. A modification of the method is suggested to assay the fibrinolytic activity.

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