Experimentally induced cholera in guinea pigs. I. Elaboration of the method of infection
Kotenok, I.F.; Urakov, N.N.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 11: 84-87
1976
ISSN/ISBN: 0372-9311 PMID: 1007732 Document Number: 98783
The method of intrapulmonary infection of guinea pigs was suggested for the assessment of the virulent properties of cholera vibrios. Addition into the diluent of 10% peptone, 10% gelatine and 0.05% agar-agar led to the reduction of LD50 by over 1000 times. A specific infectious process coursing in an acute generalized form with bacteriemia and affection of the small intestine developed in the infected animals. The majority of the animals perished in 1 to 2 days.