Changes in the cytochemical properties of chromatin after stimulating proliferation in a stationary culture of Chinese hamster cells
Terskikh, V.V.; Kir'ianova, E.A.; Zosimovskaia, A.I.; Zelenin, A.V.
Tsitologiia 18(9): 1085-1089
1976
ISSN/ISBN: 0041-3771 PMID: 1034988 Document Number: 96986
The first part of the prereplicative period in stimulated for cell proliferation steady-state culture of Chinese hamster cells was studied cytochemically. Investigation of the binding pattern of basic dye Acridine orange and Actinomycin D with cell nuclei and the melting profile of chromatin in situ suggests that one of the early events occurring in the course of stimulation of cell proliferation in steady-state culture is chromatin activation, i.e. changes in its physicochemical properties resulting in augmentation of transcription.