Stimulating effect of pancreatic DNase on Bacillus subtilis growth as a function of the physiologic properties of the inoculation culture
Kupriianova, F.G.; Egorov, S.I.; Musina, L.G.
Mikrobiologiia 57(5): 745-750
1988
ISSN/ISBN: 0026-3656 PMID: 3150518 Document Number: 321662
The stimulating effect of pancreatic DNAse on Bacillus subtilis growth was studied in relation to the content of "slowly growing" cells in the inoculation culture in the phase of decelerated growth. Three cell fractions of B. subtilis were obtained using the stepwise separation of the population in terms of buoyant density in the phase of decelerated growth. In contrast to fractions II and III, fraction I contained cells with decelerated growth, competent, permeable to exogenous DNAase I, and sensitive to the action of this enzyme. The faster growth of bacterial cells in fraction I was shown to be associated with the shorter lag period of these cells having a longer generation time.