Antiproliferative activity of protocatechualdehyde on Chinese hamster cells grown in culture
Nishimura, M.; Umeda, M.; Kusanagi, A.; Urakabe, R.; Yukimatsu, K.; Otawara, S.
Gan 72(6): 868-879
1981
ISSN/ISBN: 0016-450X PMID: 7341336 Document Number: 181383
The mode of cytotoxic action of protocatechualdehyde on V79 cells was examined. The cytotoxicity of protocatechualdehyde on V79 cells was time- and cell cycle-dependent. The lethal action is selective for the cells at S phase only. The cells lethally damaged at S phase seemed to complete their DNA synthesis, although the rate of DNA synthesis became lower and the duration of S phase was prolonged. Some cells arriving at G2 phase directly entered the next S phase without cell division and died of karyorrhexis or karyolysis. Cells treated at stages of the cell cycle other than S showed normal progression of their cell cycle to the G1/S boundary. The block point of the cell cycle seemed to lie a little behind that with hydroxyurea. When the cells at phases other than S were blocked with protocatechualdehyde for 14 hr, elongation of the next S phase occurred after removal of protocatechualdehyde. On alkaline sucrose gradient analysis, little scission of parental DNA was observed in the cells treated with protocatechualdehyde.