Duration of the mitotic cycle of Chinese hamster cells cultured at 30--39 degrees
Rudneva, S.V.; Sushkov, F.V.; Polikarpova, S.I.; Portugalov, V.V.
Biulleten' Eksperimental'noi Biologii i Meditsiny 82(11): 1365-1367
1976
ISSN/ISBN: 0365-9615 PMID: 1035117 Document Number: 99567
The duration of the mitotic cycle (T) at temperatures of 30, 33, 36 and 39 degrees C was studied in subline 237 of Chinese hamster cells with the aid of the radioautographic method. T was the least at 39 degrees C and increased with reduction of the cultivation temperature. At the temperature range of 33--39 degrees C prolongation of T and its periods was "proportional" to the temperature under study. The characteristic curve gradient of T dependence on the temperature showed a sharp change in the direction of greater figures with the temperature reduction from 33 to 30 degrees C. Analysis of the results of other studies on T duration of the human amniotic cells demonstrated that such sharp elevation of the duration of the mitotic cycle occurred with the change from 39 to 40--41 degrees C. The G1 period was the most and G2--THE LEAST Sensitive to changes of the cultivation temperature.