Stopping of spontaneous beating of cultured mouse and rat myocardial cells by a toxin (thermostable direct hemolysin) from Vibrio parahaemolyticus
Goshima, K.; Honda, T.; Takeda, Y.; Miwatani, T.
Recent Advances in Studies on Cardiac Structure and Metabolism 11: 615-620
1976
ISSN/ISBN: 0363-5872 PMID: 1031961 Document Number: 96695
Low concentrations of thermostable direct hemolysin from Vibrio parahaemolyticus stopped the spontaneous beating of cultured mouse and rat myocardial cells. These low concentrations depolarized the maximal diastolic potential and inhibited the generation of action potential of cultured myocardial cells. The toxin lost its activity when preincubated with ganglioside, GT1 or GM1. GT1 was more effective than GM1. High concentrations of the toxin caused morphological damage of cultured mouse and rat myocardial cells, but did not stop the beating, or cause morphological damage of cultured chick myocardial cells.