A method of isolating Vibrio parahaemolyticus from different substrates
Ibragimov, F.K.; Pogorelova, N.P.; Boĭko, A.V.; Burkin, V.S.; Gerok, G.I.
Laboratornoe Delo 5: 71-72
1990
ISSN/ISBN: 0023-6748 PMID: 1695981 Document Number: 349381
A method for isolation of parahemolytic vibrios is suggested, based on their high mobility in semiliquid agar gel. A special semiliquid medium was designed and tried, containing agar-agar, peptone, sodium chloride, mannose, gelatin, indicator, Progress agent. The material is inoculated into the center of a petri dish. After incubation at 37 degrees C parahemolytic vibrios form macrocolonies on the surface of the medium, colored black against the red background of the medium. This method permits ruling out preliminary enrichment of the inoculation material in liquid medium, is more effective and rapid.