Collection of Vibrio parahaemolyticus species members: phenotypic and genotypic characteristics
Rykovskaia, O.A.; Smolikova, L.M.; Monakhova, E.V.; Chemisova, O.S.; Podoĭnitsyna, O.A.; Golenishcheva, E.N.; Sanamiants, E.M.; Sagakiants, M.M.; Dalikova, R.R.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 2014(6): 3-9
2014
ISSN/ISBN: 0372-9311 PMID: 25816506 Document Number: 673276
Formation of Vibrio parahaemolyticus collection according to modern methodical opportunities and understanding of causative agent biology. Traditional biochemical tests and PCR-testing of species-specific genes were used to confirm species membership. Catalase, DNAse, proteolytic and tweenase activity was determined by common methods. Virulence was evaluated by a complex method: hemolytic activity was determined in Kanagawa test (KT), urease--in Christensen medium, PRC-testing of tdh and-trh genes. Serotyping was carried out with a commercial O/K-sera kit. PCR-genotyping was carried out by marker genes of 7 pathogenicity islands (VPaI-1-7). Species membership was confirmed for the studied strains. Serologic typing allowed to detect members of 18 serologic groups among the collection strains. All the collection cultures were divided into 4 groups based on KT-Ure-tdh-trh features recombination. A number of genetic variants were detected, strains belonging to a pandemic group and O3:K6 serogroup were determined. A collection of V. parahaemolyticus cultures was formed and characterized by a large set of pheno- and genotypic features. A database was developed including information on strain origins, pheno- and genetic features, with genetic variants given, for ease of use of the collection.