Biofilm formation by strains of Burkholderia cepacia complex in dependence of their phenotypic and genotypic characteristics
Shaginian, I.A.; Danilina, G.A.; Chernukha, M.Iu.; Alekseeva, G.V.; Batov, A.B.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 2007(1): 3-9
2007
ISSN/ISBN: 0372-9311 PMID: 17523420 Document Number: 614436
Biofilm formation was studied in 54 strains of Burkholderia cepacia complex isolated in 7 Moscow hospitals. 80% of strains (biofilm groups I and II) had the capacity to biofilm formation and only 16.7% of strains (group III) were not capable to, biofilm formation. Molecular genetic methods allowed to identify one of the epidemic markers (CBL, IS hybrid sequence Burkholderia Cepacia,,Epidemic Strain Marker - BCESM), in 46.7, 23.3, and.33.3% of strains from group I, II, and III respectively. Gene cepR from the Quorum Sensing system was identified in three biofilm groups in nearly equal frequency (92.3, 96.2 and 100% for group I, II, and III respectively), whereas cepl gene was found more often in group I (76.9%) and II (65.4%). Strains from all three groups had protease and lipase activity and 13.3% of group I strains had chitinolytic activity. B. cepacia strains from group I produced hemolysin in 33.3% of cases, from group II - in 26.6%, and from group III - in 11.1% of cases. The majority of Moscow hospital strains of B. cepacia complex were identified as B.cenocepacia (genomovar III, group A). RAPD-PCR method enabled to differentiate isolated strains into several genotypic variants. 13.3% of strains from group I were susceptible to imipenem/ciprofloxacin, as well as 33.3% of isolates from group II and 44.4% of isolates from group III.