Hydrolysis of biological materials for histamine determination on the isolated guinea-pig ileum
Dabrowski, R.
Acta Physiologica Polonica 26(3): 317-319
1975
ISSN/ISBN: 0044-6033 PMID: 1180073 Document Number: 94739
Conditions of hydrolysis of biological material for determinations of histamine (Hi) using the guinea-pig ileum method were investigated. Overheated vapour has been applied at varying intervals and it was found that optimal conditions for tissue He liberation are obtained after 2 hour hydrolysis in autoclave. Results were compared with the orginal Code's method.
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