Top Abstract Purchase Related
Home
>
Node
>
Subnode

Determination of riboflavin kinase activity in yeast

Shavlovsky, G.M.; Kashchenko, V.E.

Ukrains'kyi Biokhimichnyi Zhurnal 47(4): 536-541

1975

ISSN/ISBN: 0041-610X
PMID: 174262
Document Number: 93050
It is established that the main reason of the riboflavin kinase (RFK, EC 2.7.1.26) low specific activity in the cell-free extracts of the yeast Pichia guillermondii Wickerham ATCC 9058 is the presence of alkaline phosphatase (EC 3.1.3.1), effectively destructing flaven mononucleotide. By chromatography of the cell-free extracts of P. guillermondii on DEAE-Sephadex A-50, CM-Sphadex C-50, CM-cellulose, Sephadexes G-75 and G-100 RFK and alkaline phosphatase may be separated completely. Any of these procedures results in a several times increase of the RFK activity as compared with the initial preparation. One failed to obtain a similar effect by fractionation of the extracts with amminium sulphate and by hydroxylapatite chromatography. A simple method is developed for determining the activity of RFK in the cell-free extracts of yeast on the basis of negative adsorption of this enzyme on DEAE-Sephadex A-50. A selective inhibition of alkaline phosphatase by ions Be2+ and F- yields a less satisfactory result. The data are presented on the PFK activity of certain species of flavinogenic (Pichia guillermondii, Torulopsis camdida) and non-flavinogenic (Pichia ohmeri, Candida utilis, Saccharomyces cervisiae) yeast.

Document emailed within 1 workday
Secure & encrypted payments

Sibirnyĭ, A.A.; Shavlovskiĭ, G.M.; Ksheminskaia, G.P.; Orlovskaia, A.G. 1977: Riboflavin transport in cells of riboflavin-dependent yeast mutants Mikrobiologiia 46(2): 376-378
Shabalin, I.A.; Vagabov, V.M.; Tsiomenko, A.B.; Zemlenukhina, O.A.; Kulaev, I.S. 1977: Polyphosphate kinase activity in yeast vacuoles Biokhimiia 42(9): 1642-1648
Sajnaga, E.; Kubiński, K.; Szyszka, R. 2008: Catalytic activity of mutants of yeast protein kinase CK2alpha Acta Biochimica Polonica 55(4): 767-776
Hübner-Woźniak, E.; Bułhak-Jachymczyk, B. 1979: Colorimetric method of determination of the erythrocyte glutathione reductase activity and its use in the enzymatic test for detection of riboflavin deficiency Roczniki Panstwowego Zakladu Higieny 30(4): 349-358
Shavlovskiĭ, G.M.; Ksheminskaia, G.P.; Romaniuk, V.M. 1970: A cytosine deficient mutant of the yeast Candida guilliermondii capable of synthesizing large quantities of riboflavin Mikrobiolohichnyi Zhurnal 32(3): 281-286
Shieh, H.L.; Hansen, H.; Zhu, J.; Riedel, H. 1996: Activation of conventional mammalian protein kinase C isoforms expressed in budding yeast modulates the cell doubling time--a potential in vivo screen for protein kinase C activators Cancer Detection and Prevention 20(6): 576-589
Ronca-Testoni, S.; Lucacchini, A. 1981: Determination of adenosine kinase activity by high-pressure liquid chromatography Italian Journal of Biochemistry 30(3): 190-200
Jaros-Kamińska, B.; Opacka, J.; Slaska, K.; Ciesielska, E.; Szmigiero, L.; Gniazdowski, M. 1977: Attempts at transcription of yeast nuclear and mitochondrial DNA. II. Template activity of yeast DNA Medycyna Doswiadczalna i Mikrobiologia 29(1): 35-42
Elkins, B.N. 1977: Determination of creatine kinase isoenzyme activity by anion-exchange column chromatography and centrifugal analyzer Clinical Chemistry 23(8): 1510
Bekzhanova, E.E.; Takhirov, M.T. 1993: Determination of riboflavin in reservoir water using a fluorescent method Gigiena i Sanitariia 6: 72-73
Garcia-Bustos, J.F.; Marini, F.; Stevenson, I.; Frei, C.; Hall, M.N. 1994: PIK1, an essential phosphatidylinositol 4-kinase associated with the yeast nucleus EMBO Journal 13(10): 2352-2361
Szyszka, R.; Lopaczyński, W.; Gałasiński, W.; Grankowski, N.; Gasior, E. 1986: Further studies on the quaternary structure of yeast casein kinase Ii Acta Biochimica Polonica 33(1): 39-46
Kodentsova, V.M.; Vrzhesinskaia, O.A.; Risnik, V.V.; Sokol'nikov, A.A.; Spirichev, V.B. 1994: Isolation of riboflavin-binding apoprotein from chicken egg protein and its use for determining riboflavin in biological samples Prikladnaia Biokhimiia i Mikrobiologiia 30(4-5): 603-609
Korobko, I.V.; Korobko, E.V.; Chupikova, N.I.; Vanechkin, M.A.; Smirnova, E.V.; Kiselev, S.L.; Georgiev, G.P. 2002: The use of the two-hybrid cloning in yeast for functional characterization of protein kinase MAK-V Molekuliarnaia Biologiia 36(3): 491-495
Sutton, A.; Freiman, R. 1997: The Cak1p protein kinase is required at G1/S and G2/M in the budding yeast cell cycle Genetics 147(1): 57-71
Bystrykh, L.V.; Trotsenko, I.A. 1983: Purification and several properties of dihydroxyacetone kinase from the methylotrophic yeast Candida boidinii Biokhimiia 48(10): 1611-1616
Li, Y.Q.; Huang, X.Z.; Xu, J.G.; Chen, G.Z. 1992: Synchronous spectrofluorimetry for simultaneous determination of riboflavin and pyridoxine and its application in multivitamin preparations Yao Xue Xue Bao 27(1): 52-56
Vlasova, I.I.; Kuprin, S.P. 1992: Evaluation of distances in yeast phosphoglycerate kinase molecules using saturation effect ESR spectra at 77 K Biofizika 37(5): 927-934
Timchenko, A.A.; Tsiuriupa, S.N. 1982: Large-scale structural changes of yeast phosphoglycerate kinase molecule upon substrate binding Biofizika 27(6): 1017-1021
Woollard, D.C.; Indyk, H.E. 2002: Rapid determination of thiamine, riboflavin, pyridoxine, and niacinamide in infant formulas by liquid chromatography Journal - Association of Official Analytical Chemists 85(4): 945-951