Synchronous spectrofluorimetry for simultaneous determination of riboflavin and pyridoxine and its application in multivitamin preparations
Li, Y.Q.; Huang, X.Z.; Xu, J.G.; Chen, G.Z.
Yao Xue Xue Bao 27(1): 52-56
1992
ISSN/ISBN: 0513-4870 PMID: 1529715 Document Number: 393972
A new method for the simultaneous determination of riboflavin and pyridoxine by synchronous spectrofluorimetry is described. The measurement was carried out in a buffer solution of pH 7 with .DELTA.lambda. = 58 nm for synchronous scanning. Under the experimental conditions two peaks appeared at 526 nm and 389 nm in the obtained synchronous fluorescence spectrum, corresponding to riboflavin and pyridoxin, respectively. This method was proved to be simple and specific. The fluorescence relative intensity is linearly related with concentration over the range of 0 .apprx. 1 .mu.g/ml and 0 .apprx. 1.5 .mu.g/ml for riboflavin and pyridoxine, respectively. The detection limit was 0.5 ng/ml for riboflavin and 1 ng/ml for pyridoxin. The relative errors of the measurements were less than 5% for artifical samples. The proposed method has been applied to the analyses of riboflavin and pyridoxine in three kinds of commercial multivitamin preparations. The obtained synchronous spectra of multivitamin preparations coincided with those of the mixed standard solution of riboflavin and pyridoxine. Recoveries of 93.5 .apprx. 105.7% were obtained.