Determination of adenosine kinase activity by high-pressure liquid chromatography
Ronca-Testoni, S.; Lucacchini, A.
Italian Journal of Biochemistry 30(3): 190-200
1981
ISSN/ISBN: 0021-2938 PMID: 6268570 Document Number: 182637
High-pressure liquid chromatography (reverse-phase mode) was used to assay adenosine kinase in cell and tissue extracts. The method is optimized for a rapid and selective analysis using 6-methylthiopurine riboside as substrate, isocratic elution and detection at 300 nm. A complete separation of substrate and product is achieved in about 3 min with no interference by other UV-absorbing compounds; the limit of detection is 20 pmoles.
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