An improved buffer system for use in single-column gradient-elution ion-exchange chromatography of amino acids
Murren, C.; Stelling, D.; Felstead, G.
Journal of Chromatography 115(1): 236-239
1975
ISSN/ISBN: 0021-9673 PMID: 1202067 Document Number: 87451
Separation of amino acids was achieved by Thomas et. al. by producing on the ion-exchange column a pH gradient derived from the accurate mixing of 2 buffers of high and low pH with equimolar Na concentration. The technique as 1st reported relied on a citrate-phosphate buffer system which apparently gives somewhat limited control of the pH gradient during elution of the basic amino acids. An alternative citrate-borate buffer system is described which gives better control of the pH gradient in the basic region with a resulting improvement in the separation of the appropriate amino acids.