Separation of oligopeptides and amino acids by chromatography on the ion-exchange resin, Chelex X-100
Boisseau, J.; Jouan, P.
Journal of Chromatography 54(2): 231-237
1971
ISSN/ISBN: 0021-9673 PMID: 5545783 Document Number: 34647
An improved method is described for separation of peptides and alpha -amino acids using the Chelex X-100 exchanger in the Cu2 + form. After equilibration of the resin with ammonia solution, pH 10.3, acidic and neutral peptides and acidic amino acids are eluted with water. The neutral amino acids and basic peptides are eluted with 1.5 M NH4OH.
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