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Separation of serum creatine kinase isoenzymes by ion-exchange column chromatography

Klein, B.; Foreman, J.A.; Jeunelot, C.L.; Sheehan, J.E.

Clinical Chemistry 23(3): 504-510

1977

ISSN/ISBN: 0009-9147
PMID: 837538
Document Number: 123614
We describe a practical, technically convenient DEAE-Sephadex chromatographic column and a three-fraction salt-gradient elution procedure, with which serum creatine kinase activity is rapidly and quantitatively separated into the isoenzyme components with excellent analytical recovery of the total activity applied. The homogeneity of the isoenzyme fractions was demonstrated by electrophoresis and rechromatography. Sera assessed by the Mercer fractionation [Clin. Chem. 20, 36 (1974)] and the present procedure showed excellent agreement.

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Elkins, B.N. 1977: Determination of creatine kinase isoenzyme activity by anion-exchange column chromatography and centrifugal analyzer Clinical Chemistry 23(8): 1510
Bostick, W.D.; Denton, M.S.; Dinsmore, S.R. 1980: Liquid-chromatographic separation and on-line bioluminescence detection of creatine kinase isoenzymes Clinical Chemistry 26(6): 712-717
Van Steirteghem, A.C.; Zweig, M.H.; Schechter, A.N. 1978: Radioimmunoassay of creatine kinase isoenzymes in human serum: isoenzyme MM Clinical Chemistry 24(3): 414-419
Linke, M.; Menzel, K.; Wiedemann, G. 1984: The effect of neonatal acidosis on the activity of serum creatine kinase and its brain-specific isoenzymes in the serum of newborn infants on their first day of life Kinderarztliche Praxis 52(5): 232-235
Chen, C.F.; Lien, I.N.; Lu, F.J. 1983: Serum creatine kinase activity and its isoenzymes in Duchenne muscular dystrophy Journal of the Formosan Medical Association 82(2): 265-273
Tarkkanen, P.; Komor, S.; Cornely, C.; Hacke, W.; Greiling, H. 1979: Bioluminescence assay of creatine kinase and its isoenzymes in serum and cerebrospinal fluid Clinical Chemistry 25(9): 1644-1648
Rao, P.S.; Evans, R.G.; Mueller, H.S. 1977: Mechanism of protection and activation of creatine kinase isoenzymes by dithiothreitol in human serum Clinical Chemistry 23(10): 1948-1949
Panteghini, M.; Pagani, F. 1987: Isoforms of creatine kinase isoenzymes in serum in acute myocardial infarction after intracoronary thrombolysis Clinical Chemistry 33(11): 2039-2042
Damour, O.; Meriénne, M.; Raichvarg, D.; Fauchier, P. 1979: Creatine kinase isoenzymes: interest in their joint determinations in cerebrospinal fluid and serum in various neurological diseases Annales de Biologie Clinique 37(6): 373-374
Hamlin, C.; Ackerman, E. 1978: Relative merits of two electrophoretic and two column-chromatographic kets for determining serum creatine kinase isoenzyme MB activity Clinical Chemistry 24(11): 2013-2017
Bhargava, A.S.; Preus, M.; Khater, A.R.; Günzel, P. 1990: Effect of iloprost on serum creatine kinase and lactate dehydrogenase isoenzymes after isoprenaline-induced cardiac damage in rats Arzneimittel-Forschung 40(3): 248-252
Shoda, T.; Ueda, S. 1978: Separation of serum creatine phosphokinase isoenzymes in various disease by agar gel electrophoresis ; CPK i type (BB type) isoenzyme in serum of a cerebrovascular disease patient Rinsho Byori. Japanese Journal of Clinical Pathology 26(5): 441-445
Hassanaly, F.; Desch, G.; Martin, B.; Charrier, N.; Rabanit, A.; Soutif, A.; Autran, P. 1986: Simultaneous presence of isoenzymes CK-BB and CKm of serum creatine kinase in small-cell bronchopulmonary cancer. Limitations of the use of M subunit immuno-inhibition technics Annales de Biologie Clinique 44(4): 412-414
Szász, G.; Kinne, E.; Colombo, J.P.; Gruber, W. 1979: Influence of indicating enzyme reaction on apparent creatine kinase activity creatine kinase in serum, VII Journal of Clinical Chemistry and Clinical Biochemistry 17(11): 689-691
Gerhardt, W.; Waldenström, J.; Hörder, M.; Hofvendahl, S.; Billström, R.; Ljungdahl, R.; Berning, H.; Bagger, P. 1982: Creatine kinase and creatine kinase B-subunit activity in serum in cases of suspected myocardial infarction Clinical Chemistry 28(2): 277-283
Nealon, D.A. 1985: Relative inhibition of human adenylate kinase and creatine kinase isoenzymes by adenosine 5'-monophosphate and diadenosine pentaphosphate Clinical Chemistry 31(2): 333-334
Matsumoto, K.; Kikuchi, H.; Kano, S.; Iri, H.; Takahashi, H.; Umino, M. 1988: Automated determination of drugs in serum by liquid chromatography with column-switching. I. Separation of antiepileptic drugs and metabolites Clinical Chemistry 34(1): 141-144
Arai, K.; Mori, M.; Hironaga, T.; Itabashi, H.; Tanaka, K. 2012: Ion chromatographic separation of inorganic ions using a combination of hydrophilic interaction chromatographic column and cation-exchange resin column Se Pu 30(4): 404-408
Ottaviani, F.; Pagani, E. 1976: Determination of CPK isoenzymes by column chromatography Quaderni Sclavo di Diagnostica Clinica E di Laboratorio 12(3): 373-391
Schifferli, J. 1983: Clinical importance of creatine kinase isoenzymes Schweizerische Rundschau für Medizin Praxis 72(18): 605-608