Temperature-gradient DNA probe chromatography of nucleic acids on nonporous supports
Suyama, A.; Tsuchiya, A.; Fukuda, M.; Yano, K.
Nucleic Acids Symposium Series 27: 23-24
1992
ISSN/ISBN: 0261-3166 PMID: 1289817 Document Number: 390239
Temperature-gradient DNA probe chromatography of nucleic acids on a nonporous support with a homogeneous particle size of 2.5 microns showed a higher base sequence discriminating power and a larger linear capacity than that on a porous support with a larger and less homogeneous particle size. The resolution on the nonporous support was high enough to separate samples with a single-base mismatch of the less destabilizing groups, including G-G and G-T base mismatches, even when it locates very near the end of the probe.