Mass Culturing of Ditylenchus dipsaci to Yield Large Quantities of Inoculum

Faulkner, L.R.; Bower, D.B.; Evans, D.W.; Elgin, J.H.; Faulkner, L.R; Bower, D.B; Evans, D.W; Elgin, J.H

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Mass Culturing of Ditylenchus dipsaci to Yield Large Quantities of Inoculum

Faulkner, L.R.; Bower, D.B.; Evans, D.W.; Elgin, J.H.

Journal of Nematology 6(3): 126-129

1974

ISSN/ISBN: 0022-300X

PMID: 19308113

Document Number: 79736

The authors describe a method for rearing large numbers of Ditylenchus dipsaci on lucerne tissues in a short period of time and with little contamination. Reproduction was best in darkness at 20 to 25 deg C. Maximum numbers were obtained at 3 to 6 weeks after which they declined rapidly.