Effect of Estrogens on the Fluorescence Intensity of Catecholamines in the Limbic-Hypothalamic Structures of the Brain
Timofiychuk, I.; Savchuk, T.; Shvets, V.; Slobodian, K.; Slobodian, O.
Georgian Medical News 2018(282): 150-156
2018
ISSN/ISBN: 1512-0112 PMID: 30358560 Document Number: 696015
In scientific works of recent years, there is much evidence that sex hormones are actively involved in the processes of neurogenesis, synaptogenesis. Electron microscopy data indicate the presence of membrane estrogen receptors in limbic-hypothalamic structures, which confirms the role of sex hormones in learning and memory processes. In the basis of neurochemical mechanisms of the development of cognitive disorders, in addition to the hormonal component, a leading role belongs to noradrenergic and serotonergic neurotransmission. The purpose of our study is to establish the effect of hypoestrogenia on the level of catecholamines of the limbic-hypothalamic system of the brain. The study was carried out on female rats. Animals were divided into three groups: false-operated, with eryniectomy, with no estrous cycles. Ovariectomy was performed according to the method of Ya.M. Kabak. The determination of monoamines was carried out by the Falk-Ovman method in the modification of A.Yu. Budantsev in the limbic-hypothalamic structures of the brain and expressed in conventional units. Statistically significant changes were considered for p <0.05. Ovariectomy caused a decrease in the level of catecholamines in the limbic-hypothalamic structures of the brain, but fluorescence rates were higher than in animals with aging. The age-related decrease in the fluorescence of catecholamines was observed in all the studied structures of the brain. In the nuclei of the hypothalamus and in the CA3 field of the hippocampus, there was a more significant decrease in the fluorescence level of catecholamines, which may indicate the presence of some desynchronoses.