The cardiac injury effect of microwave radiation on rabbit and its mechanism
Zhu, W.; Shen, N.; Zhong, X.; Hou, J.; Lü, S.; Cai, J.
Wei Sheng Yan Jiu 44(5): 818-821
2015
ISSN/ISBN: 1000-8020 PMID: 26591782 Document Number: 685461
To investigate the cardiac injury effect of different intensities microwave radiation on rabbits and its possible mechanism. Rabbits were radiated by intensity of 50, 100, 150 and 200 mW/cm2 2450 MHz microwave for 20 min. 6 h after microwave radiation, the heart tissue was taken. ATP and mitochondria complex IV and V were measured in myocardial cells. The changes of myocardial tissue were observed by light microscopic. The expression of Caspase-3 and HSP 70 were detected by western blotting. The activity of ATP and mitochondria complex IV and V decreased significantly compared with normal control in cardiac tissue. 100, 150 and 200 mW/cm2 microwave radiation group vs. control group (P <0. 05). The HE staining result showed that myocardial cell appears edema, muscle fiber malalignment, cells appeared obvious injury. Results of western blotting showed that the expression of Caspase-3 and HSP 70 protein increased significantly in different dosage radiation group (P <0. 05). Microwave radiation has injury effect on rabbit heart. The possible mechanism may be related with inducing cell apoptosis by changing of stress level in myocardial cell.