Ion-exchange properties of cell walls of red seaweed Phyllophora crispa
Meĭchik, N.R.; Popova, N.I.; Nikolaeva, I.I.; Ermakov, I.P.; Kamnev, A.N.
Prikladnaia Biokhimiia i Mikrobiologiia 47(2): 194-200
2011
ISSN/ISBN: 0555-1099 PMID: 22808744 Document Number: 655247
Research into ion-exchange properties of cell walls isolated from thallus of red seaweed Phyllophora crispa was carried out. Ion-exchange capacity and the swelling coefficient of the red alga cell walls were estimated at various pH values (from 2 to 12) and at constant ionic strength of a solution (10 mM). It was established that behavior of cell walls as ion-exchangers is caused by the presence in their matrix of two types of cation-exchange groups and amino groups. The amount of the functional group of each type was estimated, and the corresponding values of pK(a) were calculated. It can be assumed that ionogenic groups with pK(a) -5 are carboxyl groups of uronic acids, and ionogenic groups with pK(a) -7.5 are carboxyl groups of the proteins. Intervals of pH in which cation-exchange groups are ionized and can take part in exchange reactions with cations in the environment are defined. It was found that protein was a major component of cell wall polymeric matrix because its content was 36%.