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Enzynatic method of disrupting yeast cell walls

Konovalov, S.A.; Vorotilo, S.P.; Sokolova, L.B.

Prikladnaia Biokhimiia i Mikrobiologiia 11(4): 620-622

1975

ISSN/ISBN: 0555-1099
PMID: 1208420
Document Number: 91358
The influence of the preparation of mucolytic enzymes obtained from the culture liquid filtrate of Actinomyces griseinus on the yeast Candida guilliermondii was investigated. Lipids, hydrocarbons and proteins were extracted and free amino acids were separated from the biomass made of disrupted yeast cells. The extract contained 73% of cytoplasmic substances whereas normal yeast (enzymically untreated) contain on the average 13.7%. Lipids and hydrocarbons were measured.

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